Figure 1.

Small molecule kinase inhibitors (Dasatinib, CK59, and CID755673) decrease NK cell degranulation and cytokine release in primary human PBMCs. PBMCs were treated with (A) Dasatinib, directed against Src family kinases; (B) CK59, targeting CaMKII; and (C) CID755673, inhibiting PKD family kinases and subsequently incubated with K562 target cells at 37°C for 2 (degranulation) and 6 (cytokine release) hours, respectively. Cells were mixed with respective fluorochrome-conjugated mAbs and the frequency of degranulating or cytokine producing NK cells was determined via flow cytometry. Data represent values relative to corresponding DMSO control. Comparison of every inhibitor-treated group to its corresponding DMSO control group was performed by Wilcoxon test (*P < 0.05,**P < 0.01,***P < 0.001). Values represent mean of the indicated number of donors ±SD.