Figure 2.

Donor-specific responses of pure NK cells treated with Dasatinib, CK59, and CID755673. Purified NK cells were treated with (A) Dasatinib, directed against Src kinases; (B) CK59, targeting CaMKII; and (C) CID755673, inhibiting PKD family kinases and subsequently incubated with K562 target cells at 37°C for 2 (degranulation) and 6 (cytokine release) hours, respectively. Cells were mixed with respective fluorochrome-conjugated mAbs and the frequency of degranulating or cytokine producing NK cells was determined via flow cytometry. Data represents values relative to corresponding DMSO control. Comparison of every inhibitor-treated group to its corresponding DMSO control group was performed by Wilcoxon test (*P < 0.05,**P < 0.01,***P < 0.001). Values represent mean of the indicated number of donors ±SD.