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. Author manuscript; available in PMC: 2013 Mar 18.
Published in final edited form as: Head Neck. 2009 Oct;31(10):1263–1273. doi: 10.1002/hed.21103

Figure 4. Effect of sequential treatment on apoptosis and cell cycle.

Figure 4

A–C. Apoptotic rates in Tu212, Tu177, and SqCC/Y1 cells upon sequential treatment. Z→ DP consistently shows a lower apoptotic rate than the DP combination. D. Immunoblotting for apoptosis-related markers in sequentially treated Tu212 cell line. Z→DP shows less cleavage of PARP and caspases than DP, DPZ or DP→Z drug treatments (black arrows, pre-cleaved; white arrows, cleaved bands). E. Representative graph of SqCC/Y1 cells treated for 24 hours with DP, Z, DPZ, DP→ Z, or Z→DP. DP treatment induced S and G2/M arrest while Z induced G0/G1 arrest. The cell cycle distributions by DP, DPZ and DP→Z were similar. When Z was given first, there was an increase in G1 arrest (gray line: the control).