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. Author manuscript; available in PMC: 2013 Mar 18.
Published in final edited form as: Mol Cancer Ther. 2009 Jan;8(1):178–184. doi: 10.1158/1535-7163.MCT-08-0643

Figure 2.

Figure 2

gp120-IIIB-induced apoptosis. PC3 and DU145 cell lines and PrEC cells were treated with 100 ng/mL gp120-IIIB (solid columns) or 100 ng/mL gp120-IIIB + 1 µg/mL anti-CXCR4 antibody (open columns) in 100 µL PBS and incubated in 5% CO2 overnight (16 h). The percent increase in the number of membrane-permeable cells or cells undergoing chromatin condensation compared with untreated controls was measured by flow cytometry that was done in triplicate in three separate experiments. ★, P < 0.01, statistical significance in the percent increase of apoptotic cells after gp120-IIIB treatment or decrease in cell permeability or chromatin condensation between cells treated with gp120-IIIB and cells treated with gp120-IIIB + anti-CXCR4 antibody.