Figure 4. Increased energy expenditure in Egr-1^−/− mice.

(a) Food intake of wild-type and Egr-1^−/− mice (n = 6) at first week and ninth week of feeding. (b) Fat absorption of wild-type and Egr-1^−/− mice (n = 6) measured on week 9 of feeding. Fat absorption = ([food intake × food lipid content]-[stool output × stool lipid content])/(food intake × food lipid content) × 100. (c) Rectal temperature was measured at week 9 of feeding in chow-fed or high-fat fed mice. (d) Oxygen consumption (VO₂) and (e) carbon dioxide production (VCO₂) were measured by metabolic cage system (CLAMS, Columbia Instrument) during a period of three days after 16 week of high-fat feeding (n = 5). Values from nighttime and daytime were presented. Values are expressed as the mean ± SD. (*, P < 0.001). (f) Physical activity was measured with CLAMS. The average movement counts during the nighttime and daytime were presented.