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. 2013 Mar 18;3:1476. doi: 10.1038/srep01476

Figure 6. Egr-1 null WAT expressed some BAT-specific genes under HF diet feeding.

Figure 6

(a) Expression of β1-AR, PGC1α, PGC1β and UCP1 in WAT from both wild-type (WT) and Egr-1 null mice (KO) under both chow or HF diet feeding was analyzed by QPCR. Values are normalized by 18S. (b) Expression of UCP1 protein in WAT from both wild-type (WT) and Egr-1 null mice (KO) under HF diet feeding was analyzed by Western blotting with UCP1 antibody (Sigma). (c) Fold changes of UCP1 protein in WAT high-fat fed mice were determined by densitometry and normalized to β-actin.