Table 2.
Effect of native ghrelin and BIM compounds on the number of GFP-positive cells, number of cFos-positive nuclei, number, and percentage of GFP-positive cells expressing cFos protein in the ArcN in NPY-Renilla GFP mice.
| GFP-positive cells | cFos-positive nuclei | GFP-positive cells expressing cFos | % GFP-positive cells expressing cFos | |
|---|---|---|---|---|
| Vehicle (n = 5) | 78.2 ± 10.5 | 6.0 ± 2.1 | 1.0 ± 0.4 | 1.5 ± 0.7 |
| Ghrelin (n = 4) | 106.0 ± 9.5 | 35.7 ± 11.4* | 14.8 ± 5.8 | 13.9 ± 4.9 |
| BIM-28131 (n = 4) | 84.5 ± 9.4 | 43.5 ± 7.6** | 18.0 ± 4.9* | 20.7 ± 3.9** |
| BIM-28163 (n = 4) | 102.5 ± 14.9 | 24.2 ± 7.5 | 11.5 ± 4.1 | 11.9 ± 3.7 |
| Ghrelin + BIM-28163 (n = 4) | 81.2 ± 23.1 | 30.2 ± 8.6* | 16.0 ± 7.7* | 23.4 ± 7.5** |
Coronal sections of the ArcN at approximately 2.0–1.9 mm anterior to the interaural line were quantified unilaterally. Data represent mean ± SEM.
*P < 0.05 and **P < 0.01 vs. vehicle.