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Replication of IBV-M41 in 3T3–DC-SIGN cells. 3T3 or 3T3–DC-SIGN cells were infected with 200 μl virus inocula of allantoic fluid dilutions of IBV-M41 with titer of 108.3 EID50/ml for 1 h at 37 °C. 8 h post infection, total RNA was extracted from cells and was subjected to RT-PCR with primers targeting to IBV nucleocapsid N gene or host GAPDH gene. Anti-sense RNA was used to derive negative strand specific cDNA.
