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. Author manuscript; available in PMC: 2013 Aug 5.
Published in final edited form as: Nat Commun. 2013 Feb 5;4:1436. doi: 10.1038/ncomms2429

Figure 3. Nested Dlx expression in the pharyngeal arches of paddlefish.

Figure 3

Viewed laterally, wholemount in situ hybridization in stage 34 P. spathula embryos reveals expression of (a,b) Dlx1-2, (c,d) Dlx5-6, and (e,f) Dlx3-4 in progressively more ventrally-restricted domains in the hyoid and gill arches, resulting in a nested Dlx code in these arches, schematized in (g). Frontal views of the same embryos reveal similarly nested expression patterns of (h,i) Dlx1-2, (j,k) Dlx5-6 and (l,m) Dlx3-4 in the mandibular arch, schematized in (n). Sections reveal that paddlefish pharyngeal arch expression of (o-u) Dlx1-6 is restricted to the neural crest-derived mesenchyme, and excluded from the ectoderm, endoderm and mesodermal core. 1-3, gill arches 1-3; e, eye; ect, ectoderm; end, endoderm; h, hyoid arch; m, mandibular arch; mes, mesoderm; mth, mouth; olf, olfactory organ; nc, neural crest-derived mesenchyme; o, otic vesicle. Scale bars: (a-f) = 200μm; (h-m) = 200μm; (o-t) = 50μm.