Table 2.
Primers of 9 candidate house-keeping genes used in qRT-PCR
| Gene namea | Primer sequenceb | Amplicon size (bp) | Slopec | R2d | Efficiencye |
|---|---|---|---|---|---|
|
ACT1 |
F-CAAAGCCAACAGGGAGAAGATGAC |
105 |
−3.131 |
0.991 |
2.086 |
| R-CGACCAGAAGCGTACAAGGAGAG | |||||
|
ACT2 |
F- TTCTGATTCCGTGAGGATTTTG |
149 |
−3.313 |
0.985 |
2.004 |
| R- GTGAGGTGGATGTTCGTAGGG | |||||
|
ARF1 |
F- CGGTGCTGGTAAAACGACAA |
135 |
−3.131 |
0.964 |
2.086 |
| R- TGACCTCCCAAATCCCAAAC | |||||
|
ARF4 |
F- GTGCTCGTGAACCATGTGAA |
140 |
−3.130 |
0.997 |
2.087 |
| R- AACCTCCAATCCCTCGTGAA | |||||
|
EF1α |
F- CAGGGCAAGGTTTGAAAGATAA |
111 |
−3.216 |
0.972 |
2.046 |
| R- CGTCTGCTTTGCGATTGAG | |||||
|
RP4 |
F- AAAGAAACGAGCATTGCCCTTCCG |
119 |
−3.317 |
0.984 |
2.002 |
| R- TTGTCGCTGACACTGTAGGGTTGA | |||||
|
RP18 |
F- TAGAATCCTCAAAGCAGGTGGCGA |
133 |
−3.158 |
0.976 |
2.073 |
| R- AGCTGGACCAAAGTGTTTCACTGC | |||||
|
TBP1 |
F- ATGTCAAGCAGAAAGTCAAGAATCC |
173 |
−3.204 |
0.988 |
2.054 |
| R- GCCGTAATATCCCTAACTCCCAAG | |||||
|
TBP2 |
F- AGCGAGGAAGACTCCAGGTTG |
171 |
−3.018 |
0.957 |
2.144 |
| R- ACTACTGAAAGAACTGTGAGTGAGC |
a: ACT1 and ACT2, Actin; ARF1 and ARF4, ADP-ribosylation factor; TBP1 and TBP2, TATA box binding protein; RP4 and RP18, ribosomal protein; EF1α, translation elongation factor 1α. b, Primer sequence of RP4 and RP18 were adopted from Zhu et al. (2011) [29]. c, Slope value of the standard curve. d, Regression coefficient calculated from the regression line of the standard curve. e, Real-time qRT-PCR efficiency calculated by the standard curve method.