Table 1.
Analysis method parameters
| Analysis method | Multiplex PCR group 1 | Multiplex PCR group 2 |
|---|---|---|
| Peak detector tab |
|
|
| Peak detection algorithm |
Advanced |
Advanced |
| Size calling method |
Local Southern |
Local Southern |
| Minimum peak half width |
4 points and |
2 points |
| Allele tab |
|
|
| Mono cut-off value |
0.95 |
N/A |
| Range filter |
|
|
| Blue |
146-194 |
181-250 |
| Green |
136-159 |
151-199 |
| Yellow | 116-174 | 131-174 |
Optimized parameters for automated specific peak detection, precise sizing, and accurate allele identification.