Table 2.
Marker parameters
| Multiplex PCR reaction | Markers | Minimal size | Maximum size | Marker repeat |
|---|---|---|---|---|
| 1 |
BAT25 |
115 |
145 |
1 |
| 1 |
BAT26 |
105 |
135 |
1 |
| 1 |
D3S3623 |
200 |
230 |
2 |
| 1 |
D5S346 |
70 |
115 |
2 |
| 1 |
D6S262 |
160 |
190 |
2 |
| 1 |
D7S481 |
175 |
205 |
2 |
| 2 |
D2S123 |
200 |
240 |
2 |
| 2 |
D3S1262 |
120 |
150 |
2 |
| 2 |
D9S171 |
100 |
130 |
2 |
| 2 |
D17S250 |
175 |
210 |
2 |
| 2 | D18S61 | 145 | 180 | 2 |
Defined size ranges of markers, which include the GDB human genome database population distribution and additional allele sizes observed in our studies for optimal automatic allele identification.