Table 1.

Oligonucleotide specifications.

Name	Used for	(5′–3′)	Position*	Target	Reference
EUB338	FISH	GCTGCCTCCCGTAGGAGT	338	Most Bacteria	Amann et al. (1990)
EUB338 II	FISH	GCAGCCACCCGTAGGTGT	338	Most Planctomycetales	Daims et al. (1999)
EUB338 III	FISH	GCTGCCACCCGTAGGTGT	338	Most Verrucomicrobiales	Daims et al. (1999)
NEU653	FISH	CCCCTCTGCTGCACTCTA	653	Most halophilic and halotolerant	Wagner et al. (1995)
Competitor NEU653	FISH	TTCCATCCCCCTCTGCCG		Nitrosomonas spp
NTSPA712	FISH	CGCCTTCGCCACCGGCCTTCC	712	Most members of the phylum Nitrospirae	Daims et al. (2001)
Competitor NTSPA712	FISH	CGCCTTCGCCACCGGTGTTCC		Nitrospirae
NTSPN693	FISH	TTCCCAATATCAACGCATTT	693	Nitrospina gracilis	Juretschko (2000)
616F	PCR	AGAGTTTGATYMTGGCTCAG	8	Bacteria	Juretschko et al. (1998)
630R	PCR	CAKAAAGGAGGTGATCC	1529	Bacteria	Juretschko et al. (1998)
NTSPA1158R	PCR	CCCGTTMTCCTGGGCAGT	1158	Most Nitrospira	Maixner et al. (2006)
NSE87F	PCR	AGTGGCGAACGGGTGAGGAATA	87	Most Nitrospira	This study
NSE1124R	PCR	TCTCTCCAGAGTGCCCGGCATGA	1124	Most Nitrospira	This study
610IIF	Sequencing	GTGCCAGCAGCCGCGGT	479	Most bacteria	Ustinova et al. (2001)
M13F	Sequencing	GTAAAACGACGGCCAG	Region flanking cloning site	pGEMT easy vector	–
M13R	Sequencing	CAGGAAACAGCTATGA	Region flanking cloning site	pGEMT easy vector	–

^*E. coli numbering.