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. 2013 Feb 18;12:18. doi: 10.1186/1475-2859-12-18

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Copyright ©2013 Manabe et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Growth characteristics of strains MGB874 and 874ΔrocG producing the alkaline cellulase Egl-237. Strains MGB874 (open circles) or MGB874∆rocG (closed circles) were transformed with pHYS237 for production of alkaline cellulase Egl-237. The strains were cultured in 2xL medium containing 7.5% (w/v) maltose monohydrate by batch fermentation with a 30-L jar fermentor. Cell yield (A), extracellular cellulase activity (B), external pH of the growth media (C) and ammonia concentration in the growth media (D) were measured at the indicated times. Arrows indicate the point at which transcriptome analyses were conducted.