Table 3.

Bacterial strains and plasmids used in or constructed for this study

Strain or plasmid	Relevant properties†	Source or reference
Strain
Bacillus subtilis
168	trpC2	[4]
168∆rocG	trpC2 ∆rocG::spec	This study
MGB874	trpC2 ∆prophage1-6 ∆PBSX ∆SPβ ∆pks ∆skin ∆pps∆ (ydeK-ydhU) ∆(yisB-yitD) ∆(yunA-yurT) ∆(cgeE-yodU) ∆(ypqP-ypmQ) ∆(yeeK-yesX)∆(pdp-rocR) ∆(ycxB-sipU) ∆(yrkS-yraK) ∆(sboA-ywhH) ∆(yyb-yyaJ) ∆(yncM-yndN)	[6]
874∆rocG	MGB874 ∆rocG::spec	[9]
Escherichia coli
HB101	supE44 ∆(mcrC-mrr) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 leuB6 thi-1	Takara Bio
Plasmid
pHY300PLK	Shuttle vector for E. coli and B. subtilis	Takara Bio
pHYS237	pHY300PLK carrying the gene for alkaline endo-1,4-β-glucanase (Egl-237) from Bacillus sp. strain KSM-S237, containing amp and tet	[6]

^†Antibiotic resistance genes are abbreviated as follows: amp, ampicillin; tet, tetracycline; spec, spectinomycin; neo, neomycin.