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. Author manuscript; available in PMC: 2013 Mar 18.
Published in final edited form as: Cell Physiol Biochem. 2012 Jul 3;30(2):395–406. doi: 10.1159/000339033

Fig. 2.

Fig. 2

Volume change measured with fluorescence quenching. (a) The change of cell volume in response to hypotonic and hypertonic perfusion. (b) The change of cell volume in response to shear stress. Both data sets were obtained from fluorescence micrographs of MDCK cells loaded with 4 μM calcein. The arrows in (a) indicates the solution change from isotonic (320 mOsm) to hypotonic (168 mOm) or hypertonic solution (429 mOsm). The arrow in (b) indicates the change of the shear stress from 0.7 to 7.0 dyn/cm2. The plot in (b) is the average of relative intensity changes obtained from ten different locations in each of the micrographs. The error bar indicates the standard deviation.