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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Muscle Res Cell Motil. 2012 Jul 1;33(5):305–312. doi: 10.1007/s10974-012-9304-y

Table 2.

Dissociation constants of MANT-ADP from myosin and actomyosin, as determined in Fig. 5.

Construct Kd, myosin (μM) Kd, actomyosin (μM)
S619C 0.8 ± 0.1 1.3 ± 0.3
S619C-IQ 1.1 ± 0.5 1.1 ± 0.4
T688C 0.3 ± 0.1 0.9± 0.3
T688C/678C 2.5 ± 0.6 9.2 ± 4.1
M761 1.0 ± 0.3 30 ± 15
M761-2R 2.1 ± 0.2 ≥180
Muscle S1 0.27 ± 0.05 ≥160