Figure 4.
Adult-specific rescue of zyd mutants by conditional transgene expression rapidly reduces seizure susceptibility. Expression of UAS-zyd was controlled with the heat-inducible hsp70-Gal4 driver that is not expressed at room temperature and is activated above 30°C. zyd1 mutants were reared at 22°C until adulthood (2 d posteclosion), then exposed to a brief 37°C heat pulse. TS seizure susceptibility was strongly reduced in zyd1 mutants 6 h after transgene expression with hsp70-Gal4 (red bars). Genetically identical controls not receiving a heat pulse exhibited seizures at all time points (blue bars). n = 30 flies/time point.