Figure 10.

Actin disarrangement impacts HIV-1 infection. Macrophages were treated with IL-27 (50 ng/ml), cytochalasin A (5 µM), or cytochalasin D (5 µM), or transfected with control or SPTBN1 siRNA (50 pmol). F-actin was labeled with fluorescein phalloidin and nuclei were stained with DAPI. Bars, 10 µm. Cells were infected with HIV-LUC-V and luciferase activity was measured 4 d after infection. Data shown represent means ± SE of three independent experiments.