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. 2013 Mar 11;210(3):517–534. doi: 10.1084/jem.20120572

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Figure 3. — I-Mac lacks a cellular host factor to support HIV-1 infection. (A) Heterokaryons were formed between M-Mac and I-Mac. M-Mac was stained with CellTracker Green and I-Mac was stained with CellTracker Red. Double-stained heterokaryons were yellow as indicated by arrows. Bar, 50 µm. (B, left) Double-stained heterokaryons between M-Mac and I-Mac were sorted by FACS with the indicated gate. (middle) M-Mac and I-Mac were mixed without fusion. (right) Heterokaryons were reanalyzed by FACS to confirm purity after sorting. (C) Sorted heterokaryons were infected with HIV-LUC-V. HIV-1 infection of heterokaryons between M-Mac and I-Mac was compared with infection levels of M-Mac or I-Mac homokaryons. Data shown represent mean ± SE of three independent experiments.