Figure 6.

OT-I cells primed in the presence of IL-1 retain their phenotype upon boost 8 wk later. 10⁴ OT-I and 10⁴ OT-II cells were injected i.p. into UBC-GFP C57BL/6 mice. 6 d later, the mice were immunized with OVA and LPS with or without IL-1. 8 wk after priming, the mice were boosted with OVA and LPS. Three days later, cells from liver and lung were stimulated for 4 h with PMA and ionomycin. The percentage of granzyme B⁺ and IFN-γ⁺ cells among the OT-I cells in a representative animal is shown in A and summary cell number and percent of granzyme B⁺ and IFN-γ⁺ cells are shown in B and C. This experiment was performed three times with similar results.