Table 2.
Output for relative expression of EFG1 and ALS3 genes by use of ΔΔCt method (REST©, 2008 V2.0.7). Results indicate that the differences between EFG1 expression in the yeast of control and sample groups are significant as well as the expression of ALS3 gene.
| Gene | Type | Reaction Efficiency | Expression | Standard Error | 95% C.I. | P(H1) * | Result |
|---|---|---|---|---|---|---|---|
| ACT1 | REF | 1.0 | 1.000 | ||||
| EFG1 (unrelated siRNA) | TRG | 1.2 | 1.046 | 0.636 - 1.820 | 0.482 - 2.277 | 0.638 | |
| EFG1 (500 nM siRNA) | TRG | 1.2 | 0.357 | 0.209 - 0.674 | 0.153 - 0.858 | 0.000 | DOWN● |
| ALS3 (500 nM siRNA) | TRG | 0.8 | 0.122 | 0.066 - 0.288 | 0.049 - 0.347 | 0.000 | DOWN |
| EFG1 (1,000 nM siRNA) | TRG | 1.0 | 0.179 | 0.101 - 0.394 | 0.080 - 0.487 | 0.000 | DOWN |
| ALS3 (1,000 nM siRNA) | TRG | 0.8 | 0.037 | 0.021 - 0.068 | 0.017 - 0.075 | 0.000 | DOWN |
*P(H1), probability of alternate hypothesis that difference between sample and control groups is due only to chance. TRG, target; REF, reference; ●down-regulation of ALS3 and EFG1 genes after using siRNA; C.I., confidence interval.