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. Author manuscript; available in PMC: 2013 Mar 18.
Published in final edited form as: Methods. 2011 Sep 8;55(4):273–280. doi: 10.1016/j.ymeth.2011.08.018

Figure 6. Fluorescence-detection size-exclusion screening of human membrane proteins solubilized from HEK 293S cells.

Figure 6

100 μL of solubilized full-length human membrane proteins (see Figure 4) were injected onto a TSK-G3000SW column equilibrated in 50 mM Hepes pH 7, 200 mM NaCl, and detergent (0.5 mM β-DDM, 0.5 mM FC 14, 40 mM β-OG) at a flow rate of 0.5 ml/min. Fluorescence detection was monitored as described in section 2.4. The void volume, included volume and free EGFP molecule are labelled for the center chromatogram.