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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Scand J Immunol. 2012 Dec;76(6):559–566. doi: 10.1111/j.1365-3083.2012.02773.x

Figure 4.

Figure 4

NK cells inhibit the in vivo expansion of Th17 cells in T-bet−/− mice.NK cells were sorted from lymphocytes pooled from spleens and lymph nodes of IFN-γ+/+RAG1−/− or IFN-γ−/−RAG1−/− mice and stimulated with LPS (10 μg/ml) and IL-15 (15 ng/ml). CD4+ T cells were sorted from AChR/CFA-primed T-bet+/+ or T-bet−/− mice and cultured under Th17 polarizing condition. 2 × 105 sorted NK cells and Th17-polarizing CD4+ T cells were passively transferred into γc−/− RAG1−/− mice at a ratio of 1:1. (A) Percentages of proliferation of CD4+ T cells were analysed by FACS at day 15 after cell transfer (Mice were injected with bromodeoxyuridine at day 14 after cell transfer). Dot plots are representative of two separate experiments. (B) 2 × 105 of CD4+ cells/well were cultured with AChR (10 μg/ml) in the presence of anti-NK1.1 (20 μg/ml) for 3 days, and IL-17 was measured by ELISA. Data shown are representative of two separate experiments (n = 3 per experiment).