Figure 2.
The stability of Cy5.5-labeled Aβ1-40 peptide in serum ex vivo. Cy5.5-labeled Aβ1-40 peptides were incubated in either phosphate buffered saline (PBS) or fetal bovine serum (FBS) at 37°C for indicated periods of time, resolved on a 16% Tricine-SDS-PAGE gel, imaged using eXplore Optix optical imager (A), and then blotted to PVDF membrane and probed with the anti-human Aβ antibody 6E10 (B). The lane C contains 1 μg unlabeled Aβ peptide; all other lanes contain ~1 μg Cy5.5-labeled Aβ peptides.