Skip to main content
. 2013 Mar 18;8(3):e59721. doi: 10.1371/journal.pone.0059721

Table 1. Sequences of Primers.

Gene Direction 5′→3′ Sequence Used for
Cvhsp40 forward GCNGARGCNTAYGANGTGCT degenerate PCR
Reward TTBGTDCCNKCCTTCCAKCC degenerate PCR
outer primer AGGCGATCAAGGTCGTGGTA 3′RACE
inner primer ACCATTCCCGAAAGAACCATCA 3′RACE
outer primer ATGGTTCTTTCGGGAATGGT 5′RACE
inner primer CCACGACCTTGATCGCCTTCTT 5′RACE
forward ATGGGTAAAGACTACTATAAAACTCTTGGG verified ORF
Reward TCAATTAGGTAGAGTGTCATACAGTATGTCTT verified ORF
forward CGGTGGTGCTGAAACATA real-time qPCR
Reward GGTGGGTCTTGAGCGTGA real-time qPCR
Cvhsp70 forward ACWGTWCCXGCTTAYTTCAA degenerate PCR
Reward ACATCRAAGGTDCCRCCGCC degenerate PCR
outer primer TGCCAGCATACTTCAACGATTC 3′RACE
inner primer TGCGATTGCTGGGCTGAACG 3′RACE
outer primer CGATACCCAGAGATAGGGGAGCAAC 5′RACE
inner primer GGATGCGAGTAGAACCTCCCACGA 5′RACE
forward ATGCCTGCCATTGGTATT verified ORF
Reward TTAGTCAACTTCTTCAACCGT verified ORF
forward GTGGGAGTGTGGCAACAAGGG real-time qPCR
Reward GTGTCCGTGAAGGCAACATAGC real-time qPCR
Cvhsc70 outer primer GTCCCTTGTCGTTGGTGATGGTG 5′RACE
inner primer CGTAGGTGGTGAAGGTCTGGGTT 5′RACE
forward AAATGACGAAAGCACCCGC verified ORF
Reward ACCTGAATAGGCAGTGGAGTGAC verified ORF
forward TTGATTTGGGAACTACATAC real-time qPCR
Reward AGTCGCTCAGTGTCTGTAAA real-time qPCR
Cvhsp90 forward GCKGAGATCGCYCAGCTKATGTC degenerate PCR
Reward GCCTTCATGATRCGYTCCATGTTGGC degenerate PCR
outer primer CGTGAGGAAGACAAAGCCAAAT 3′RACE
inner primer CCCTGCTGTATCGTTACTTCTC 3′RACE
outer primer TTGGCTTTGTCTTCCTCACG 5′RACE
inner primer CGATACAGCAGGGCGAGT 5′RACE
forward ATGCCGGAAGGAATGGATACCT verified ORF
Reward TTAATCGACTTCTTCCATACGAGACG verified ORF
forward CTCGCCCTGCTGTATCGT real-time qPCR
Reward ATCGTCAAGTGAGAACCC real-time qPCR
Cv18SrRNA forward CGCCTTTCAAGATACCAAAATACGCC real-time qPCR
Reward TAGCTCTTTCTTGATTCGGTGGGTG real-time qPCR