Table 1. Sequences of Primers.
Gene | Direction 5′→3′ | Sequence | Used for |
Cvhsp40 | forward | GCNGARGCNTAYGANGTGCT | degenerate PCR |
Reward | TTBGTDCCNKCCTTCCAKCC | degenerate PCR | |
outer primer | AGGCGATCAAGGTCGTGGTA | 3′RACE | |
inner primer | ACCATTCCCGAAAGAACCATCA | 3′RACE | |
outer primer | ATGGTTCTTTCGGGAATGGT | 5′RACE | |
inner primer | CCACGACCTTGATCGCCTTCTT | 5′RACE | |
forward | ATGGGTAAAGACTACTATAAAACTCTTGGG | verified ORF | |
Reward | TCAATTAGGTAGAGTGTCATACAGTATGTCTT | verified ORF | |
forward | CGGTGGTGCTGAAACATA | real-time qPCR | |
Reward | GGTGGGTCTTGAGCGTGA | real-time qPCR | |
Cvhsp70 | forward | ACWGTWCCXGCTTAYTTCAA | degenerate PCR |
Reward | ACATCRAAGGTDCCRCCGCC | degenerate PCR | |
outer primer | TGCCAGCATACTTCAACGATTC | 3′RACE | |
inner primer | TGCGATTGCTGGGCTGAACG | 3′RACE | |
outer primer | CGATACCCAGAGATAGGGGAGCAAC | 5′RACE | |
inner primer | GGATGCGAGTAGAACCTCCCACGA | 5′RACE | |
forward | ATGCCTGCCATTGGTATT | verified ORF | |
Reward | TTAGTCAACTTCTTCAACCGT | verified ORF | |
forward | GTGGGAGTGTGGCAACAAGGG | real-time qPCR | |
Reward | GTGTCCGTGAAGGCAACATAGC | real-time qPCR | |
Cvhsc70 | outer primer | GTCCCTTGTCGTTGGTGATGGTG | 5′RACE |
inner primer | CGTAGGTGGTGAAGGTCTGGGTT | 5′RACE | |
forward | AAATGACGAAAGCACCCGC | verified ORF | |
Reward | ACCTGAATAGGCAGTGGAGTGAC | verified ORF | |
forward | TTGATTTGGGAACTACATAC | real-time qPCR | |
Reward | AGTCGCTCAGTGTCTGTAAA | real-time qPCR | |
Cvhsp90 | forward | GCKGAGATCGCYCAGCTKATGTC | degenerate PCR |
Reward | GCCTTCATGATRCGYTCCATGTTGGC | degenerate PCR | |
outer primer | CGTGAGGAAGACAAAGCCAAAT | 3′RACE | |
inner primer | CCCTGCTGTATCGTTACTTCTC | 3′RACE | |
outer primer | TTGGCTTTGTCTTCCTCACG | 5′RACE | |
inner primer | CGATACAGCAGGGCGAGT | 5′RACE | |
forward | ATGCCGGAAGGAATGGATACCT | verified ORF | |
Reward | TTAATCGACTTCTTCCATACGAGACG | verified ORF | |
forward | CTCGCCCTGCTGTATCGT | real-time qPCR | |
Reward | ATCGTCAAGTGAGAACCC | real-time qPCR | |
Cv18SrRNA | forward | CGCCTTTCAAGATACCAAAATACGCC | real-time qPCR |
Reward | TAGCTCTTTCTTGATTCGGTGGGTG | real-time qPCR |