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. 2013 Mar 18;8(3):e59147. doi: 10.1371/journal.pone.0059147

Figure 3. CSC population in the cells encapsulated in PEGDA gel.

Figure 3

MCF7 cells were encapsulated in PEGDA gels with 5 kPa modulus and cultured in stem cell culture medium. Cells before encapsulation (a), 3 days (b), 8 days (c) and 11 days (d) after encapsulation were stained with CD44−FITC and CD24-PE antibodies. The population of CD24+, CD44+ and CD44+/CD24− cells was determined by flow cytometry. Flow cytometry was repeated multiple times on each sample to ascertain reproducibility of the results.