Skip to main content
. 2013 Mar 18;8(3):e59632. doi: 10.1371/journal.pone.0059632

Figure 1. Resveratrol and verbascoside effects on IL-8 expression in normal human keratinocytes.

Figure 1

Chemical structure of resveratrol (A) and verbascoside (B). (C, D) Quantitative real-time RT-PCR time-dependent changes of IL-8 transcript. Cells were treated with 50 µM resveratrol (Resv) or verbascoside (Verb) for the indicated intervals. Keratinocytes were pre-treated with the polyphenols for 1 h before addition of TNFα (50 ng/ml), and analyzed at the indicated time-points. (E) Profile of IL-8 protein accumulation in cell supernatants measured by specific ELISA. *P<0.05 and § P<0.01 versus controls at the same time-point without polyphenol (−). (F) ELISA quantification of IL-8 in the medium of human keratinocytes treated with escalating concentration of Resv for 24 h. *P<0.05 and § P<0.01 versus controls without polyphenol (0 µM). (G) Quantitative real-time RT-PCR measurement of IL-8 mRNA stability. After 12 h treatment with TNFα, the RNA polymerase II inhibitor DRB (75 µM) was added for the indicated time-points. Data are expressed as the mean ± S.D. of three consecutive determinations in three independent experiments.