Figure 6. Resveratrol did not protect phosphorylated EGFR from endogenous phosphatases, led to EGFR accumulation in the keratinocyte membranes, and induced its cytosolic degradation.

Western blot analysis of the plasma membrane fraction, where cadherin was used as a loading control (A) and of the cytosolic fraction (C). After 24h treatment with resveratrol (Resv), keratinocytes were stimulated with TGFα (50 ng/ml) for 10 minutes. Then, 2 µM PD168393 (PD16) were added to the medium for further 10 minutes. (B, D) Quantification of Western blot bands by densitometry. *P<0.05 and ^§ P<0.01 versus untreated controls.