Figure 1. Cromoglycate, nedocromil, dexamethasone, and human recombinant Anx-A1 inhibit IgE/anti-IgE - induced histamine and PGD₂ release from CDMCs.

CDMCs were cultured, sensitised with IgE and challenged with anti-IgE for 1h as described. The cell culture supernatant was sampled and PGD₂ release (pg/ml) or net % release of histamine was assessed by ELISA. Panels A and B. Cromoglycate 0.001–100 µM produces a concentration-dependent inhibition of histamine (panel A) or PGD₂ (panel B) release. Each data point is expressed as the mean ± SEM (n = 3). Panels C and D. Vehicle (control), nedocromil (Ned; 10nM), dexamethasone (Dex; 2nM) or human recombinant Anx-A1 protein (hr-Anx-A1; 1 nM and 10 nM) was added to the wells to test their inhibitory effect on histamine (panel C) and PGD₂ release (panel D). Each data point is expressed as the mean ± SEM (n = 3).* Signifies P<0.05; **P<0.01 and ***P<0.001 relative to vehicle treated cells.