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. 2013 Mar 18;8(3):e59115. doi: 10.1371/journal.pone.0059115

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© 2013 Qin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Structure of GhGalT1 protein. N: N-terminal; TM: transmembrane domain, GalT: the conservative galactosyltransferase functional domain; C: C-terminal. (B) Comparison of the predicted amino acid sequence of GhGalT1 with some known GalT proteins. The alignment was generated with the CLUSTAL W program. The same nucleotides are highlighted by asterisk, conserved and similar residues are indicated by colon or dot mark. (C) Phylogenetic relationships between GhGalT1 and other GalTs in plants. A neighbour-joining tree was generated with MEGA5 from 1000 bootstrap replicates. The accession numbers of these known proteins in GenBank are: Gossypium hirsutum GhGalT1 (JX448620); Populus trichocarpa PtGalT1 (XP_002330737) and PtGalT2 (XP_002317466); Arabidopsis thaliana AtbGalT1 (At1g53290) and AtbGalT2 (At3g14960); Zea mays ZmGalT1 (NP_001152267); Vitis vinifera VvGalT1 (XP_002283081); Ricinus communis RcGalT1 (XP_002524276); Glycine max GmGalT1 (XP_003543216), GmGalT2 (XP_003556804) and GmGalT3(XP_003527208); Oryza sativa OsGalT1 (Os06g0156900) and OsGalT2(CAD44839.); Hordeum vulgare HvGalT1 (BAJ94890) and HvGalT2(ABL11234); Sorghum bicolor SbGalT1 (XP_002436508).