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. 2013 Mar 18;8(3):e59115. doi: 10.1371/journal.pone.0059115

Figure 10. RT-PCR analysis of expression of the cell wall-associated genes in the GhGalT1 overexpression transgenic Arabidopsis plants.

Figure 10

The expression levels of genes involved in the biosynthesis of cellulose in primary cell wall (CesA1, CesA3 and CesA6), cellulose in secondary cell wall (CesA4, CesA7 and CesA8), xyloglucan (XXT1, XXT2 and XXT5), xylan (FRA8 and IRX12), pectin (GAUT1, GAUT8, GAUT9, Qua2 and xgd1) and lignin (4CL and CCoMoAT1) were examined in two-week-old seedlings of GhGalT1 transgenic lines, wild type and mutants, using ACTIN2 as an internal control. (A) Semi-quantitative RT-PCR analysis. (B) Quantitative RT-PCR analysis. Error bars represent standard errors of three replicates. Asterisk represents (very) significant difference (one asterisk: P value<0.05; two asterisk: P value<0.01) between the transgenic lines/mutant and wild-type by t-test. WT: wild type; GhGalT1oe-L1 and -L5: GhGalT1 overexpression transgenic line 1 and 5; SALK043252 and SALK015338: two T-DNA insert mutants. The assays were repeated three times along with three independent repetitions of the biological experiments.