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. 2013 Mar 18;8(3):e59625. doi: 10.1371/journal.pone.0059625

Figure 2. ICV leptin failed to increase pSTAT3 in OXT neurons of the PVN belonging to the hypophyseal system.

Figure 2

Upper sets of panels depict low magnification photomicrographs of the anterior, medial and posterior parts of the PVN of fasted rats subjected to intraperitoneal FG injection, ICV leptin treatment and further triple NPI, FG and pSTAT3 staining. Right column of images shows merge of NPI (red fluorescent staining), FG (green fluorescent staining) and pSTAT3 (brown staining) signals. Bottom set of panels show high magnification photomicrographs of area marked in low magnification images. Numbered arrows point to dual-labeled cells as follows: 1-double pSTAT3/FG-IR cell, negative for NPI; 2-double pSTAT3/NPI-IR cell, negative for FG; 3-double FG/NPI-IR cell, negative for pSTAT3. Scale bars: 50 µm (Upper panels), 20 µm (Bottom panels).