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. 2013 Mar 18;8(3):e59625. doi: 10.1371/journal.pone.0059625

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© 2013 Perello, Raingo

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Left and right upper sets of panels depict low magnification photomicrographs of the anterior, medial and posterior parts of the PVN in DIO rats treated with vehicle or leptin, respectively. Red fluorescent and brown signals represent NPI-IR and pSTAT3-IR, respectively. Cells with co-localization of NPI-IR with pSTAT3-IR are seen in the merge panels. Bottom set of panels show high magnification photomicrographs of area marked in low magnification images, with arrows pointing to dual-labeled cells. Scale bars: 50 µm (Upper panels), 20 µm (Bottom panels).