Figure 6. ICV leptin affected OXT gene expression in the PVN (A) and required OXT signaling to regulate body weight (B) in fasted DIO rats.

A. OXT mRNA levels are presented as relative to levels observed in PVN of control fed rats. B. Body weight was determined after completion of 48 h fasting and simultaneous ICV administrations, every 6 h, of either vehicle or leptin in presence or absence of the OXT receptor antagonist. *, p<0.05. **, p<0.01. n.s., not significant.