Figure 2. Th1 cytokine expression one week after last immunization. Fresh lymphocytes from all mice were isolated one week following the final immunization and analyzed for Th1 cytokines, TNFα, IFN-γ and IL-2. (A) Gating strategy shows TNFa, IFN-gamma and IL-2 from CD4⁺/CD8⁺ T cells. (B) Cytokines data for SEA (medium alone values subtracted) is enumerated per immunization group and shown for CD4⁺ and CD8⁺ T-cells. Data was acquired on an LSRI instrument analyzed with flowjo software. *p < 0.05 per the unpaired t test.