Figure 3. Effect of adjuvants on T cell proliferations. Balb/c mice were immunized with pcD-VP1 with cytokine adjuvants intramuscularly in the prime-boost fashion as indicated on x-axis. T cells were isolated from animals (3 per group) and stimulated with VP1 protein as specific antigen, ConA as positive control, or BSA as negative control. Proliferation was analyzed by the MTT dye method and expressed as a stimulation index.