Figure 5. Effect of adjuvants on the cytokine expressions in macrophages. RNAs were extracted macrophages derived from immunized mice at the 48h after the first immunization. The expression of the cytokines IFN-α, IL-6, TNF-α, TGFβ, IL-10 and co-stimulatory molecule CD86 were analyzed by RT-PCR and run on 1% agarose gel (left panel) and relative concentrations to HPRT were plotted (right panel).