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. 2012 Nov 1;8(11):1659–1667. doi: 10.4161/hv.22105

Table 2. Target anti-apoptotic gene primers and parameters of the PCR.

Target gene Primers Parameters
HPRT
5′: 5′-GTTGGATACAGGCCAGACTTTGTTG-3′
3′: 5′-GAGGGTAGGCTGGCCTATGGCT-3′
94°C 30s, 60°C 30s, 72°C 40s
Spi2A
5′: 5′-CCCCTTTGACCCGAATGA-3′
3′: 5′-CTTCCTGAGTTGGGCATC-3′
94°C 30s, 53.5°C 30s, 72°C 40s
BCL-XL
5′: 5′-CGATGAGTTTGAACTGCG-3′
3′: 5′-CACCTAGAGCCTTGGATCC-3′
94°C 30s, 55°C 30s, 72°C 40s
BCl-2
5: 5′-GGCTACGAGTGGGATGCT-3′
3′: 5′-GGGTCATGTGTGTGGAGAG-3′
94°C 30s, 55°C 30s, 72°C 40s
IL-7Ra
5”: 5′-CCGATCCATTCCCCATAACG-3′
3′: 5′-ATGAGGTGAAAGGCGTTGAA-3′
94°C 30s, 56°C 30s, 72°C 40s
IL-15Ra 5′: 5′-GCTCCAGGCTGACACCATC-3′
3′: 5′-TCTCAGCCGTGCCGTGT-3′
94°C 30s, 62.5°C 30, 72°C 40s