Table 2. Target anti-apoptotic gene primers and parameters of the PCR.
Target gene | Primers | Parameters |
---|---|---|
HPRT |
5′: 5′-GTTGGATACAGGCCAGACTTTGTTG-3′ 3′: 5′-GAGGGTAGGCTGGCCTATGGCT-3′ |
94°C 30s, 60°C 30s, 72°C 40s |
Spi2A |
5′: 5′-CCCCTTTGACCCGAATGA-3′ 3′: 5′-CTTCCTGAGTTGGGCATC-3′ |
94°C 30s, 53.5°C 30s, 72°C 40s |
BCL-XL |
5′: 5′-CGATGAGTTTGAACTGCG-3′ 3′: 5′-CACCTAGAGCCTTGGATCC-3′ |
94°C 30s, 55°C 30s, 72°C 40s |
BCl-2 |
5: 5′-GGCTACGAGTGGGATGCT-3′ 3′: 5′-GGGTCATGTGTGTGGAGAG-3′ |
94°C 30s, 55°C 30s, 72°C 40s |
IL-7Ra |
5”: 5′-CCGATCCATTCCCCATAACG-3′ 3′: 5′-ATGAGGTGAAAGGCGTTGAA-3′ |
94°C 30s, 56°C 30s, 72°C 40s |
IL-15Ra | 5′: 5′-GCTCCAGGCTGACACCATC-3′ 3′: 5′-TCTCAGCCGTGCCGTGT-3′ |
94°C 30s, 62.5°C 30, 72°C 40s |