Figure 6. Depletion of Foxp3⁺ Treg provokes heterogeneous antitumor responses that correlate with development of vitiligo. (A–C) Groups of C57BL/6 DEREG mice (n = 4–25) were inoculated s.c. with 5 × 10⁴ B16F10 melanoma cells on day 0. On days -2, 5, 12 and 19, mice were injected i.p. with either 500 ng diphtheria toxin A (DTA) or PBS. Mice were then monitored and tumor growth was measured over 150 d. Tumor-bearing mice were categorized into 4 groups based on tumor growth pattern: Group A (PBS treated), Group B (DTA-treated, tumor outgrowth by 60 days post-inoculation), Group C (DTA-treated, tumor eliminated) and Group D (DTA-treated, tumor outgrowth 60 days post-inoculation). Tumor size (mm²) for each individual mouse was plotted and results were pooled from seven independent experiments (A). Representative pictures of mice from each group are shown in (B). The incidence of vitiligo in B16F10-bearing mice belonging to the indicated group is shown in (C). Statistical differences between mice groups were determined by Fisher’s exact tests (*p < 0.05; **p < 0.01; ***p < 0.001).