Figure 3. Alterations of the frequency T-cell subsets as induced by temolozomide in melanoma patients. (A–D) Frequency of T cell subpopulations on frozen/thawed peripheral blood mononuclear cells (PBMCs, as assessed by flow cytometry) before the administration of temolozomide (TMD) and after the first and second cycles of chemotherapy: *p < 0.05; NS = non-significant (paired Student's t-test).