Table 1.
Immune cells density in cervical intraepithelial neoplasia and squamous cervical carcinoma
| Cell densities (cells/mm2) | |||||||
|---|---|---|---|---|---|---|---|
| CD4+ | CD8+ | DP CD4+/CD8+ | CD25+ | Foxp3+ | DP CD25+/Foxp3+ | ||
| Intraepithelial compartment | |||||||
| CIN 1 | Mean | 6.04 | 19.71 | 0.35 | 0.18 | 0.38 | 0.04 |
| (n = 26) | Median | 1.00 | 13.25 | 0.00 | 0.00 | 0.00 | 0.00 |
| CV* (%) | 57.0 | 69.8 | 51.3 | 22.4 | 36.1 | 20.0 | |
| IQ Range | 8.75 | 14.38 | 0.50 | 0.00 | 0.00 | 0.00 | |
| Skewness | 2.49 | 3.11 | 2.42 | 4.31 | 3.43 | 4.42 | |
| CIN 2 | Mean | 1.20 | 15.95 | 0.19 | 0.05 | 2.02 | 0.07 |
| (n = 21) | Median | 0.5a | 13.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| CV* (%) | 55.7 | 122.7 | 64.6 | 31.7 | 43.2 | 29.9 | |
| IQ Range | 1.00 | 1.00 | 0.50 | 0.00 | 2.00 | 0.00 | |
| Skewness | 2.15 | 1.89 | 1.14 | 2.56 | 3.17 | 3.04 | |
| CIN 3 | Mean | 5.80 | 17.17 | 0.83 | 0.00 | 2.06 | 0.00 |
| (n = 25) | Median | 2a | 13.00 | 0.00 | 0.00 | 0.00 | 0.00 |
| CV* (%) | 59.5 | 125.7 | 32.1 | 0.0 | 26.9 | 0.0 | |
| IQ Range | 5.50 | 16.50 | 0.50 | 0.00 | 0.00 | 0.00 | |
| Skewness | 2.54 | 0.88 | 3.75 | 3.95 | |||
| SCC | Mean | 5.52 | 15.42 | 0.42 | 0.17 | 1.71 | 0.40 |
| (n = 24) | Median | 1.25 | 10.50 | 0.00 | 0.00 | 0.25 | 0.00 |
| CV* (%) | 45.6 | 78.4 | 54.7 | 26.9 | 42.7 | 38.4 | |
| IQ Range | 4.13 | 17.75 | 0.50 | 0.00 | 1.25 | 0.00 | |
| Skewness | 3.10 | 1.87 | 1.76 | 3.98 | 3.39 | 2.46 | |
| † P value | 0.06 | 0.67 | 0.97 | 0.41 | 0.06 | 0.13 | |
| Stromal compartment | |||||||
| CIN 1 | Mean | 30.52 | 52.65 | 0.53 | 0.80 | 6.76 | 0.30 |
| (n = 26) | Median | 7.75 | 35.75 | 0c | 0.00 | 0.00 | 0.00 |
| CV* (%) | 62.3 | 101.0 | 60.1 | 32.1 | 44.2 | 38.6 | |
| IQ Range | 22.50 | 46.63 | 0.88 | 0.50 | 4.00 | 0.00 | |
| Skewness | 1.55 | 1.68 | 1.32 | 4.10 | 2.57 | 2.57 | |
| CIN 2 | Mean | 9.45 | 42.52 | 0.62 | 0.81 | 3.06 | 0.28 |
| (n = 21) | Median | 5.25 | 34.5b | 0d | 0.00 | 1.75 | 0.00 |
| CV* (%) | 68.2 | 132.8 | 47.1 | 39.7 | 87.2 | 70.9 | |
| IQ Range | 7.63 | 30.88 | 0.50 | 0.00 | 3.25 | 0.50 | |
| Skewness | 2.06 | 1.65 | 2.12 | 2.22 | 1.12 | 0.87 | |
| CIN 3 | Mean | 20.62 | 59.93 | 3.59 | 0.08 | 10.04 | 0.10 |
| (n = 25) | Median | 7.50 | 42.00 | 1c,d | 0.00 | 0.00 | 0.00 |
| CV* (%) | 82.9 | 106.1 | 33.7 | 34.6 | 29.7 | 35.4 | |
| IQ Range | 28.00 | 55.50 | 2.00 | 0.00 | 2.88 | 0.00 | |
| Skewness | 1.43 | 1.26 | 3.68 | 2.69 | 4.07 | 2.42 | |
| SCC | Mean | 29.71 | 71.58 | 1.50 | 1.71 | 5.48 | 0.33 |
| (n = 24) | Median | 12.75 | 76b | 0.25 | 0.00 | 1.75 | 0.00 |
| CV* (%) | 82.6 | 145.3 | 58.8 | 22.1 | 41.0 | 44.6 | |
| IQ Range | 48.50 | 73.50 | 2.00 | 0.00 | 4.13 | 0.13 | |
| Skewness | 1.20 | 0.28 | 1.78 | 4.29 | 3.76 | 2.35 | |
| † P value | 0.22 | 0.28 | 0.13 | 0.22 | 0.32 | 0.32 | |
*Coefficient of variation
†Kruskal-Wallis test: comparing the counts among the histological grades in stroma and epithelium
a p = 0.0003. Benhrens–Fisher test. CD4+ in CIN 2 vs. CIN 3 in epithelium
b p = 0.06. Benhrens–Fisher test. CD8+ in CIN 2 vs. SCC in stroma
c p = 0.03. Benhrens–Fisher test. DP CD4+/CD8+ in CIN 1 vs. CIN 3 in stroma
d p = 0.06. Benhrens–Fisher test. DP CD4+/CD8+ in CIN 2 vs. CIN 3 in stroma
DP double positive staining