Fig. 2.

T cell proliferation is inhibited by culture with tumor-released Survivin. a Representative Ki-67 staining at 72 h post-stimulation with anti-CD3/anti-CD28 microbeads. b Ki-67 expression was evaluated over time in T cells cultured with FLAG-HA-Survivin. CD4⁺Ki-67⁺ and CD8⁺Ki-67⁺ cell numbers are decreased after culture with FLAG-HA-Survivin compared to control. c and d CFSE dilution post-activation is significantly reduced by Survivin in CD8⁺ and CD4⁺ T cells compared to controls at 72, 96 and 120 h. e and f BrdU incorporation is diminished in CD8⁺ and CD4⁺ T cell cultures containing Survivin. Results in a, c and e are representative of 3 independent experiments. Data in b, d, and f are Means ± SD of 3 independent experiments, n = 9. *p < 0.05, **p < 0.01, ***p < 0.001