Fig. 1.

a Well (WD) or moderately differentiated (MD), oral SCC contain CD16⁺ cells with dendritic morphology. Sections of oral SCC specimens stained by IHC with HRP-DAB detection show brown CD16⁺ cells. b Cell lines selected for the study. Primary keratinocytes HTE1163, well-differentiated HNSCC 1483, and poorly differentiated HNSCC Cal27 cells were grown in Permanox slide-mounted chambers, formalin-fixed and stained by IHC for cell cycle/proliferation marker Ki67. c Detailed schematic of experimental set-up. d Phenotypes of Donor 1 DC. Monocytes differentiated with GM-CSF+IL-4 produced the attached (DC-SIGN-low) and detached (DC-SIGN-high) subsets, evaluated by flow cytometry. Numbers represent the mean fluorescence intensity (MFI) of antibody binding. NOTE: Surface phenotypes of DC from Donors 1, 2 and 3 are compared in Table 1