Fig. 2.

Dendritic cells affect viability of detached HNSCC cells. HNSCC cells were plated alone or combined at 1:1 ratios with DC-SIGN-low or DC-SIGN-high DC and cultured for 3 days. Floating cell populations were stained with anti-CD11c–PECy5, Annexin V-FITC (AnnV-FITC) and propidium iodide (PI) and analyzed by flow cytometry. Dot plots from Donor 1 DC subsets with Cal27 (a) and with 1483 (c) are shown. CD11c was used to distinguish between live DC and SCC populations. Only the PI-positive SCC cells non-specifically stained with the DC-specific anti-CD11c antibody. (Supplementary Figure 1). Fold enrichment of total viable floating HNSCC cells (Cal27 and 1483, respectively) in the presence of each DC subset for all donors (mean ± SEM from two independent experiments for each donor/HNSCC cell line combination) relative to HNSCC cells cultured alone (b and d)