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. 2013 Mar 18;200(6):699–708. doi: 10.1083/jcb.201212058

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© 2013 Neelsen et al.

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Figure 2. — Oncogene OE deregulates the cell cycle and causes DNA breakage and bi-phasic DDR activation. (A and E) FACS analysis of DNA content (DAPI) of cells overexpressing CycE for up to 8 d and Cdc25A for up to 72 h, respectively. For additional data on the accumulation of cells with >4n DNA, see Figs. 3 B and S3 H. Data are from a single representative experiment out of four repeats. (B and F) Proliferation of cells overexpressing CycE and Cdc25A, respectively (mean + SEM, n = 4). (C and G) DNA breakage after CycE and Cdc25A OE monitored by pulse-field gel electrophoresis. (D and H) DDR activation (pCHK1, pKAP1) and total DDR proteins (CHK1, KAP1) upon OE of CycE and Cdc25A, respectively. TFIIH serves as loading control for D and the top panels of H; total KAP1 levels control for KAP1 pS284 in the bottom panels of H. Molecular weight in kD of nearest protein size marker is indicated.