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. 2013 Mar 18;200(6):839–850. doi: 10.1083/jcb.201301120

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© 2013 Breker et al.

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Figure 4. — Protein localization is dynamic under stress. (a) Two strains per condition (out of a total of 254 unique changes in protein localization) as examples for changes in predominant protein localization between growth in SD and growth under stress. (b) The only localization change that is common under all three stresses is proliferation of P-bodies. Shown are two P-body proteins, Dcp1 and Dcp2, changing localization from the cytosol to punctate foci. Broken lines represent the contour of the cell from bright-field images. (c) Foci of Dcp1 and Dcp2 colocalize with Edc3-mCherry during growth under every stress condition, which shows that these are bona fide P-bodies. (d) A schematic diagram depicting the types and numbers of changes in cellular localization observed during yeast growth in the three different stresses. Bars, 5 µm.