Reverse transcription polymerase chain reaction analysis. Reverse transcription was carried out on RNA extracted from aorta from wild type (WT) and CBS +/- mice. The expression levels of (A) MTHFR, SAHH, PRDX2, NOX3 (B) MMP1, TIMP1, MMP9, TIMP4 (C) Collagen I, Collagen IV, and Elastin were analyzed. *p<0.05 versus wild type controls, #p<0.05 versus wild type controls (n=4).