A
|
|
Gene |
Primer pair sequence (5’-3’) |
Product size (bp) |
|
Collagen - I |
GACGGGAGTTTCTCCTCGGGGTC GAGTCTCCGGATCATCCACGTC |
398 |
Collagen - IV |
ATGTCAATGGCACCCATCAC CTTCAAGGTGGACGGCGTAG |
382 |
Elastin |
AAAGCAGCAGCAAAGTTCGG ACCTGGGACAACTGGAATCC |
288 |
SAHH |
TGTTGCTTTTATGTCTCTCTGG GCTTGGCATTCTCTTAAACC |
470 |
MTHFR |
GCAATTGTGGGATGTCCTCT TTCTGAGCTTGTGCATTTGG |
570 |
MMP1 |
ACCCCAAGGACATCTACAGC CACCTTCTTTGGACTCACACC |
786 |
MMP9 |
ACCTGGATGCCGTCGTGGAC TGTGGCAGCACCAGGGCAGC |
475 |
TIMP1 |
GGGGCTTCACCAAGACCTACAC AAGAAAGATGGGAGTGGGAACA |
506 |
TIMP4 |
CGGCCAGTGCAGACCCTGCTG TGAGAGGCAGGTGGCCCCGGT |
317 |
PRDX-2 |
CTTGCCTGGTGTCGGTGGTTAGT CGGCTGAATCTGAAGTCTTGGTTTT |
278 |
NOX-2 |
ATATTTTGGAATTGCAGATGAACA ATATTGAGGAAGAGACGGTAG |
650 |
GAPDH |
ACCACAGTCCATGCCATCAC TCCACCACCCTGTTGCTGTA |
903 |
|
B
|
|
Gene |
Primer pair sequence (5’-3’) |
Product size (bp) |
|
Collagen - I |
AGAACTTTGCTTCCCAGATG CTATCTGTACCACCCCCTTG |
162 |
Collagen - IV |
GCTGCTAAGAACTTGCCTTC GGGGACAGAGAAGATGTCAC |
105 |
Elastin |
TGACAGTATAGGGCTGAGCA GAGTTGTTGTGGGTGAGACA |
238 |
SAHH |
CATGGGGTAGGAAAAGATTG ACCTCCTCACCAATGTCCTA |
144 |
MTHFR |
ACCTGAAGCATTTGAAGGAG GATAGGGCAAGAGATGCCTA |
128 |
MMP1 |
CACACTGTTCCAGCTTTACG GCTCTTGAACAGCCCATACT |
101 |
MMP9 |
CATGTCACTTTCCCTTCACC TTGCCGTCCTTATCGTAGTC |
158 |
TIMP1 |
CTCAAATGGGAGAAGCTGTT AAGTGACGGCTCTGGTAGTC |
299 |
TIMP4 |
CTGGTAAGTGAAGGGGCTAA ACCAGGTTCATGGAGGTAGA |
289 |
PRDX-2 |
TGTGCTCCATACCAGAGCTA CCTCTTCAAATGGCTTCACT |
221 |
NOX-3 |
CAGAACTTGTGTGTCCACTG CCCCTTTCTCCTTAAATTGT |
237 |
GAPDH |
TAAATTTAGCCGTGTGACCT AGGGGAAAGACTGAGAAAAC |
177 |
DNMT1 |
GGGTCTCACCAAGTATCTCA GGTGTGTGACTCCAGTTTTT |
235 |
DNMT3a |
GGGAGAGAGGGAAAATTCTA GGTTTTCTTCAAGGTTTCCT |
298 |
DNMT3b |
GACTGCCTGGAGTTCAGTAG ACAGGCAAAGTAGTCCTTCA |
285 |
MBD2 |
TAGCGCATCAGATGTAACAG AGAGACTTGTCCTGTGATGG |
199 |
H3K9 |
CTGTCTCAAAGAAGGAGGTG GGACAGACAAACACCAAAGT |
279 |
HDAC1 |
GACTGGACCCTCTGTCATTA TTTCTCCTAAAGTGCAGCTC |
248 |