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Ratiometric absorption changes of CyDPA2 and CyDPA1 with pTau and nTau titrations (0, 0.6, 0.8, 1, 2, 3, 4, 6 μg/mL) in 50 mM HEPES containing 10% DMSO (pH 7.4). Ratiometric absorption changes were obtained by measuring intensities at two wavelengths; (A) CyDPA2; at 810 nm and 750 nm; (B) CyDPA1; 630 nm and 730 nm. Error bars represent s.d. of triplicates.
